Product Description

CUL3 Polyclonal Antibody

Catalogue Numbers: BS5678-50, BS5678-100

Sizes: 50µl, 100µl

Alternative Name: Cullin-3; Cullin 3; Cullin3; CUL-3; CUL3; KIAA0617

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13618

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. CUL-1, which is the mammalian homolog of yeast Cdc53, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1-mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3, facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CUL3 polyclonal antibody detects endogenous levels of CUL3 protein.

Bioworld Molecular Weight: ~ 89 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CUL3 polyclonal antibody at 1:500 dilution
Lane 1: MCF-7 cell lysate
Lane 2: Mouse spleen tissue lysate
Lane 3: Rat spleen tissue lysate

Immunogen: Synthetic peptide, corresponding to amino acids 36-81 of Human CUL3.

Conjugate: Unconjugated

Modification: Unmodified