Product Description

CUL4A Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-19279-60, E-AB-19279-120, E-AB-19279-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: CUL4A

Target Synonym: 2810470J21Rik; AW495282; CUL 4A; CUL-4A; Cul4a; Cul4a protein; CUL4A; Cullin-4A; MGC36573; MGC64071

Research Areas: Cancer, Cell Biology

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human, Mouse

Application: WB, IHC, ELISA

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: Q13619

Background: Cullin proteins assemble a large number of RING E3 ubiquitin ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), have been identified. The two CUL4 sequences are 83% identical. They target certain proteins for degradation by binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex with DDB. They form two individual E3 ligases, DDB1-CUL4ADDB2 and DDB1-CUL4BDDB2 in this process. CUL4A appeared in both the nucleus and the cytosol, suggesting a more complex mechanism for entering the nucleus. CUL4B is localized in the nucleus and facilitates the transfer of DDB1 into the nucleus independently of DDB2.

Concentration: 1.62 mg/mL

Immunogen: Synthetic peptide of human CUL4A

Buffer: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4

Purification Method: Antigen affinity purification

Dilution: WB 1:500-1:2000, IHC 1:50-1:300, ELISA 1:5000-1:10000

Calculated MW: 88 kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only