CUL4B Polyclonal Antibody-BS7768

CUL4B Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS7768-50, BS7768-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13620

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB: 1:500~1:2000
IHC/IF: 1:50~1:200

Background: Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. Cul-1, which is the mammalian homolog of Cdc53 from yeast, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/Cul-1/F-box protein complex) consists of Skp1 associating with both Cul-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. Cul-1 mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3 facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin-like protein, where it too functions as an active component of a multifunctional E3 complex. CUL-5, or vasopressin-activated, calcium-mobilizing protein (VACM-1), is also included in the cullin family as it shares substantial sequence homology with CUL-1.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CUL4B polyclonal antibody detects endogenous levels of CUL4B protein.

Bioworld Molecular Weight: ~ 115 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of CUL4B antibody., Immunohistochemistry of paraffin-embedded rat liver using CUL4B antibody at dilution of 1:200 (400x lens)., Immunohistochemistry of paraffin-embedded mouse kidney using CUL4B antibody at dilution of 1:200 (400x lens).

Alternative Name: CUL 4B;
CUL-4B;
CUL4B;
CUL4B_HUMAN;
Cullin-4B;
DKFZp686F1470;
KIAA0695;
MRXHF2;
MRXSC;
SFM2;

Immunogen: Recombinant full length Human CUL4B.

Conjugate: Unconjugated

Modification: Unmodified