Cullin 2 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90345-50, BS90345-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q13617(Human)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IP,
All Applications: WB:1:500-1:2,000
IHC:1:50-1:200
IP:1:10-1:50
Background: Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. CUL-1, which is the mammalian homolog of yeast Cdc53, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1-mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3, facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin-like protein, where it too functions as an active component of a multifunctional E3 complex.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Specificity: Cullin 2 polyclonal antibody detects endogenous levels of Cullin 2 protein.
Bioworld Molecular Weight: 89 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Cullin 2 on PC-3M and K562 cell lysates using anti-Cullin 2 antibody at 1/1,000 dilution., Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Cullin 2 antibody. Counter stained with hematoxylin.
Alternative Name: CUL-2, CUL2, Cul2, CUL 2, CUL-2, CUL2, CUL2, cullin 2, cullin homolog 2, Cullin-2, MGC131970,
Conjugate: Unconjugated
Modification: Unmodified