Cullin-3 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90347-50, BS90347-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q13618(Human) Q9JLV5(Mouse)
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB, IHC FC
All Applications: WB:1:500-1:1,000
IHC:1:50-1:200
FC:1:50-1:100
Background: Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. CUL-1, which is the mammalian homolog of yeast Cdc53, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1-mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3, facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin-like protein, where it too functions as an active component of a multifunctional E3 complex. CUL-5, also designated vasopressin-activated, calcium-mobilizing protein (VACM-1), is also included in the cullin family as it shares substantial sequence homology with CUL-1.
Purification and Purity: Peptide affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Cullin-3 polyclonal antibody detects endogenous levels of Cullin-3 protein.
Bioworld Molecular Weight: 89 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Cullin 3 on different lysates using anti-Cullin 3 antibody at 1/1,000 dilution.
Positive control:
Lane 1: SH-SY5Y
Lane 2: 293T
Lane 3: Mouse testis tissue, ICC staining Cullin 3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: CUL-3, CUL3, CUL 3, Cul-3, cul3, CUL3, Cullin-3, Cullin3, KIAA0617, PHA2E,
Immunogen: Peptide.
Conjugate: Unconjugated
Modification: Unmodified