Product Description

CWC22 Polyclonal Antibody

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Sizes: 50µl, 100µl

Catalogue Numbers: BS65685-50, BS65685-100

Swiss-Prot: Q9HCG8

Host: Rabbit

CWC22 Polyclonal Antibody-BS65685

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,

Applications: IHC-P

All Applications: IHC-P=1:100-500

Background: Spliceosomes are multi-protein complexes that are composed of snRNPs (small nuclear ribonucleoproteins) and a variety of associated protein factors, all of which work in concert to regulate the splicing of pre-mRNA, a critical step in the post-transcriptional regulation of gene expression. CWC22 (CWC22 spliceosome-associated protein), also known as NCM, fSAPb or EIF4GL, is a 908 amino acid nuclear protein and component of the spliceosome C complex. CWC22 is associated with the spliceosome prior to catalytic steps and remains associated throughout the reaction. Belonging to the CWC22 family, CW22 contains one MI domain and a MIF4G domain. The gene encoding CWC22 maps to human chromosome 2q31.3. As the second largest human chromosome, chromosome 2 makes up approximately 8% of the human genome and contains 237 million bases encoding over 1,400 genes.

Purification and Purity: affinity purified by Protein A

Specificity: CWC22 Polyclonal Antibody detects endogenous levels of CWC22 protein.

Bioworld Molecular Weight: 105kD

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Paraformaldehyde-fixed, paraffin embedded (mouse lung tissue)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CWC22) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C,, Paraformaldehyde-fixed, paraffin embedded (rat brain tissue)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CWC22) Polyclonal Antibody, Unconjugated (bs-11464R) at 1:400 overnight at 4°C

Alternative Name: CWC22; CWC22 spliceosome associated protein homolog (S. cerevisiae); CWC22 spliceosome associated protein homolog; CWC22_HUMAN; EIF4GL; fSAPb; Functional spliceosome associated protein b; KIAA1604; NCM; Nucampholin homolog; Pre-mRNA-splicing factor CWC22 homolog.

Immunogen: KLH conjugated synthetic peptide derived from human CWC22

Modification: Unmodified

Conjugate: Unconjugated