Product Description

CX3CR1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS7449-50, BS7449-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P49238

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200

Background: Fractalkine is a transmembrane protein and chemokine involved in the adhesion and migration of leukocytes. The protein encoded by this gene is a receptor for fractalkine. The encoded protein also is a coreceptor for HIV-1, and some variations in this gene lead to increased susceptibility to HIV-1 infection and rapid progression to AIDS. Four transcript variants encoding two different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 52KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using CX3CR1 antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Immunohistochemistry of paraffin-embedded human colon carcinoma using CX3CR1 Rabbit pAb at dilution of 1:25. Perform microwave antigen retrieval with 50 mM Tris/EDTA buffer pH 8.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded rat spleen using CX3CR1 Rabbit pAb at dilution of 1:25. Perform microwave antigen retrieval with 50 mM Tris/EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Alternative Name: CX3CR1; CCRL1; CMKBRL1; CMKDR1; GPR13; GPRV28; V28

Immunogen: A synthetic peptide of human CX3CR1(NP_001328.1).

Conjugate: Unconjugated

Modification: Unmodified