Product Description

CXADR Polyclonal Antibody

Catalogue Numbers: BS5638-50, BS5638-100

Sizes: 50µl, 100µl

Alternative Name: Coxsackievirus and adenovirus receptor; CAR; Hcar; CVB3-binding protein; Coxsackievirus B-adenovirus receptor; HCVADR; CXADR; CAR

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P78310

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB ICC

All Applications: WB: 1:500~1:1000
ICC: 1:50~1:200

Background: The coxsackie and adenovirus receptor (CAR) mediates viral infection by the binding of various adenoviruses through specific protein interactions. There is a high affinity between the viral knob domain and the extracellular amino- terminal domain (designated D1) of CAR. The D1 domain alone is sufficient for knob binding in transfected cells. Determining the specific interactions between CAR and adenoviruses is imperative in order to further develop gene therapy using viral hosts. CAR is expressed in many human and murine cell types. However, cells that express CAR at low levels are not efficiently infected by adenoviruses. Possible methods of avoiding this problem in certain cell types are by either supplementing CAR or modifying the Ad knob to bind to other receptors.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CXADR polyclonal antibody detects endogenous levels of CXADR protein.

Bioworld Molecular Weight: ~ 46 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CXADR polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: NIH-3T3 cell lysate
Lane 3: H9C2 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 48-93 of Human CXADR.

Conjugate: Unconjugated

Modification: Unmodified