Product Description

CXCL10/IP-10 Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS80232-50, BS80232-100

Swiss-Prot: P02778

Host: Rabbit

CXCL10/IP-10 Polyclonal Antibody-BS80232

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: This antimicrobial gene encodes a chemokine of the CXC subfamily and ligand for the receptor CXCR3. Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Unmodification

Bioworld Molecular Weight: 11KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of THP-1, using CXCL10/IP-10 antibody at 1:3239 dilution.THP-1 cells were treated by LPS for 6 hours,Brefeldin A for 3 hours and IFNγ for 8 hours of stimulation.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Immunofluorescence analysis of NIH/3T3 cells using CXCL10/IP-10 Rabbit pAb at dilution of 1:250. Blue: DAPI for nuclear staining.

Alternative Name: CXCL10; C7; IFI10; INP10; IP-10; SCYB10; crg-2; gIP-10; mob-1

Immunogen: Recombinant fusion protein of human CXCL10/IP-10(NP_001556.2).

Modification: Unmodified

Conjugate: Unconjugated