Product Description

CXCR2 Polyclonal Antibody

Catalogue Numbers: BS65020-50, BS65020-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: P25025

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB

All Applications: WB: 1:500~1:1000

Background: IL-8 has been shown to function as a potent neutrophil chemostatic and activating peptide and is an important mediator of inflammatory diseases. Two distinct human IL-8 receptors, designated IL-8RA and IL-8RB, have been characterized. Both are expressed at a high level on neutrophils, and to a lesser extent on monocytes and myeloid cell lines. In addition, the IL-8RA subunit is expressed in T cells such as the Jurkat cell line. Both IL-8Rs are members of the seven transmembrane domain rhodopsin superfamily of receptors and as such, couple G proteins for signal transduction. The two receptors share 77% amino acid identity. IL-8RA exhibits high affinity binding for IL-8 and low affinity MGSA binding, whereas IL-8RB has high affinity binding for both IL-8 and MGSA.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CXCR2 polyclonal antibody detects endogenous levels of CXCR2 protein.

Extra Notes: Western blot (WB) analysis of CXCR2 polyclonal antibody at 1:500 dilution
Lane1:THP-1 whole cell lysate(40ug)
Lane2:A549 whole cell lysate(40ug)
Lane3:BV2 whole cell lysate(40ug)
Lane4:PC12 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 41 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: C-X-C chemokine receptor type 2;CXC-R2;CXCR-2;CDw128b;GRO/MGSA receptor;High affinity interleukin-8 receptor B;IL-8R B;IL-8 receptor type 2;CD_antigen: CD182;CXCR2;IL8RB

Immunogen: Synthetic peptide, corresponding to Human CXCR2.

Conjugate: Unconjugated

Modification: Unmodified