CXCR5 Monoclonal Antibody-BS65518

CXCR5 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65518-50, BS65518-100

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Swiss-Prot: P32302

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-P

All Applications: WB=1:500-1000 IHC-P=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

Background: The CXCR5 gene was identified as a gene specifically expressed in Burkitt's lymphoma and lymphatic tissues. CXCR5 protein is predicted to be a seven transmembrane G protein coupled receptor and belongs to the CXC chemokine receptor family. BLC, a B lymphocyte chemoattractant, was identified to be a specific ligand for this receptor. Studies of the gene and its mouse conterpart strongly suggest the essential function of the protein is in B cell migration and localization within specific anatomic compartments, such as follicles in lymph nodes as well as in spleen.

Purification and Purity: affinity purified by Protein A

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CXCR5 monoclonal Antibody detects endogenous levels of CXCR5 protein.

Bioworld Molecular Weight: ~42kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Anti-CXCR5 at 1/1000 dilution, Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CXCR5) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C., Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CXCR5) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C., Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CXCR5) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C.

Alternative Name: BLR 1; BLR1; Burkitt lymphoma receptor 1; Burkitt lymphoma receptor 1 GTP binding protein; C X C chemokine receptor type 5; C-X-C chemokine receptor type 5; CD 185; CD185; CD185 antigen; Chemokine C X C motif receptor 5; Chemokine CXC motif receptor 5; Chemokine receptor 5; CXC chemokine receptor type 5; CXC R5; CXC-R5; CXCR 5; CXCR-5; Cxcr5; CXCR5_HUMAN; MDR 15; MDR-15; MDR15; MGC117347; Monocyte derived receptor 15; Monocyte-derived receptor 15.

Immunogen: Recombinant human CXCR5

Conjugate: Unconjugated

Modification: Unmodified