Product Description

CYB5R3 (I169) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3337-50, BS3337-100

Sizes: 50µl, 100µl

Swiss-Prot: P00387

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: CYB5R3 (NADH-cytochrome b5, reductase 3, diaphorase 1) is a 301 amino acid protein encoded by the human gene CYB5R3. CYB5R3 belongs to the flavoprotein pyridine nucleotide cytochrome reductase family and has two naturally occuring isoforms. Isoform 1 is anchored to the cytoplasmic side of the endoplasmic reticulum membrane and mitochondrion outer membrane,while isoform 2 is the soluble form found in erythrocytes. CYB5R3 is involved in the desaturation and elongation of fatty acids, cholesterol biosynthesis,drug metabolism and, in erythrocytes, methemoglobin reduction.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: CYB5R3 (I169) polyclonal antibody detects endogenous levels of CYB5R3 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CYB5R3 (I169) pAb at 1:500 dilution
Lane 1: MCF-7 whole cell lysate (40ug)
Lane 2: A549 whole cell lysate (40ug)
Lane 3: The Testis tissue lysate of Rat (40ug)
Lane 4: The Testis tissue lysate of Mouse (40ug), Immunohistochemistry (IHC) analyzes of CYB5R3 (I169) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 34 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: NADH-cytochrome b5 reductase 3; B5R; Cytochrome b5 reductase; Diaphorase-1; Diaphorase1; Diaphorase 1; NADH-cytochrome b5 reductase 3 membrane-bound form; NADH-cytochrome b5 reductase 3 soluble form; DIA1

Immunogen: Synthetic peptide, corresponding to amino acids 141-190 of Human CYB5R3.

Conjugate: Unconjugated

Modification: Unmodified