Product Description

Cyclin A1/2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90352-50, BS90352-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P20248(Human) P78396(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, IHC, IP,

All Applications: WB:1:1,000-1:2,000
IHC:1:50-1:200

Background: The critical role that the family of regulatory proteins known as cyclins play in eukaryotic cell cycle regulation is well established. Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin-dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DRTF1 and E2F, and retinoblastoma protein p110. A second form of cyclin A, named cyclin A1 because of its high sequence homology to Xenopus cyclin A1, is most highly expressed in germ cells. It has been proposed that cyclin A1 can associate with Cdk2, p39 and Cdc2 p34. Cyclin A2 is a member of the highly conserved cyclin family. Cyclins regulate CDK kinases and different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin A2 is expressed in all tissues tested, in contrast to cyclin A1, which is present only in germ cells. The Cyclin A2 protein binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. Cyclin A2 also functions in the transition to DNA replication and synthesis phases of the cell cycle and is quickly destroyed as the cell moves into mitosis.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Cyclin A1/2 polyclonal antibody detects endogenous levels of Cyclin A1/2 protein.

Bioworld Molecular Weight: 52/49 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Cyclin A1/A2 on different lysates using anti-Cyclin A1/A2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: HepG2, Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cyclin A1/A2 antibody. Counter stained with hematoxylin.

Alternative Name: CCNA1, Cyclin-A, CCNA2, CCN1, CCNA, CCNA1, CCNA2, CT146, Cyclin-A, Cyclin-A1, Cyclin-A2,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified