Cyclin A2 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90353-50, BS90353-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P20248(Human) P51943(Mouse) Unigene:13094(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC
All Applications: WB:1:1,000-1:2,000
ICC:1:100-1:500
IHC:1:50-1:200
Background: The critical role that the family of regulatory proteins known as cyclins play in eukaryotic cell cycle regulation is well established. The best-characterized cyclin complex is the mitotic cyclin B/Cdc2 p34 kinase, the active component of maturing promoting factor. Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin- dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DRTF1 and E2F and retinoblastoma protein, p110. A second form of cyclin A, named cyclin A1 because of its high sequence homology to Xenopus cyclin A1, is most highly expressed in germ cells. It has been proposed that cyclin A1 can associate with Cdk2, p39 and Cdc2 p34.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Cyclin A2 polyclonal antibody detects endogenous levels of Cyclin A2 protein.
Bioworld Molecular Weight: 49 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Cyclin A2 on different lysates using anti-Cyclin A2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: PC12
Lane 2: NIH/3T3, ICC staining Cyclin A2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: Cyclin-A, CCNA2, CCN1, CCNA, Ccna2, CCNA2, Cyclin A2, Cyclin-A, Cyclin-A2,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified