Product Description

Cyclin B1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS80268-50, BS80268-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P14635

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 55KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using Cyclin B1 antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s., Immunofluorescence analysis of HeLa cells using Cyclin B1 Rabbit pAb at dilution of 1:150. Blue: DAPI for nuclear staining., Immunofluorescence analysis of NIH/3T3 cells using Cyclin B1 Rabbit pAb at dilution of 1:150. Blue: DAPI for nuclear staining., Immunofluorescence analysis of PC-12 cells using Cyclin B1 Rabbit pAb at dilution of 1:150. Blue: DAPI for nuclear staining.

Alternative Name: CCNB; CCNB1; Cyclin B1; cyclin B1

Immunogen: A synthetic peptide of human Cyclin B1(NP_114172.1).

Conjugate: Unconjugated

Modification: Unmodified