Cyclin B2 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90355-50, BS90355-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: O95067(Human) P30276(Mouse) EntrezGene:363088(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC, IP,
All Applications: WB:1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
Background: In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M-phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc 1). The Cdc/cyclin enzyme is subject to multiple levels of control of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/cyclin B enzyme and tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex . Evidence has established that B-type cyclins not only act on M-phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The two B-type cyclins, cyclin B1 and cyclin B2, have been shown to have distinct tissue distributions.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Cyclin B2 polyclonal antibody detects endogenous levels of Cyclin B2 protein.
Bioworld Molecular Weight: 45 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Cyclin B2 on different lysates using anti-Cyclin B2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: K562, ICC staining Cyclin B2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: CCNB2, ccnb2, CCNB2, CycB2, Cyclin B2, G2 mitotic specific cyclin B2, G2/mitotic specific cyclin B2, G2/mitotic-specific cyclin-B2, HsT17299, MGC108931, MGC140694,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified