Product Description

Cyclin B3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS60305-50, BS60305-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q8WWL7

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB: 1:500~1:1000
IF/ICC: 1:50~1:200

Background: Cell proliferation is controlled at specific stages of the cell cycle by distinct protein kinase complexes. These complexes consist of a catalytic subunit associating with a specific regulatory subunit to form the active kinase. The cyclins, which include cyclin A, B, C, D, E, F, G, H, I, K, L, T and their related proteins, including Dbf4, comprise the regulatory subunits of these kinase complexes. The controlled activation of the kinase complexes at various intervals of the cell cycle is regulated by the availability of the cyclins to the catalytic subunit. Unlike the catalytic subunit, which is expressed continually, the expression and stability of the regulatory subunit fluctuates depending on the stage of the cell cycle, thereby regulating kinase activity. Cyclin B3, also known as CCNB3 or CYCB3, is a 1,395 amino acid nuclear protein that belongs to the cyclin family of regulatory proteins. Expressed in testis with lower expression in a variety of other tissues, cyclin B3 is thought to be required for early meiotic prophase I, playing an important role in the meiotic cell cycle. Three isoforms of cyclin B3 exist due to alternative splicing events.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Cyclin B3 polyclonal antibody detects endogenous levels of Cyclin B3 protein.

Bioworld Molecular Weight: ~ 158 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Cyclin B3 expression in rat brain (A),
mouse liver (B) whole cell lysates., Immunofluorescent analysis of Cyclin B3 staining in C6 cells.

Alternative Name: Cyclin B3; CyclinB3; G2/mitotic-specific cyclin-B3; CCNB3; CYCB3

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cyclin B3.

Conjugate: Unconjugated

Modification: Unmodified