Cyclin C Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS75385-50, BS75385-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P24863
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB,1:500 - 1:2000
Background: The protein encoded by this gene is a member of the cyclin family of proteins. The encoded protein interacts with cyclin-dependent kinase 8 and induces the phophorylation of the carboxy-terminal domain of the large subunit of RNA polymerase II. The level of mRNAs for this gene peaks in the G1 phase of the cell cycle. Two transcript variants encoding different isoforms have been found for this gene.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Unmodification
Extra Notes: Western blot analysis of extracts of various cell lines, using Cyclin C antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Note: For research use only, not for use in diagnostic procedure.
Bioworld Molecular Weight: 33kDa
Alternative Name: CCNC; CycC; SRB11; hSRB11; cyclin-C
Immunogen: Recombinant fusion protein of human Cyclin C(NP_005181.2).
Conjugate: Unconjugated
Modification: Unmodified