Product Description

Cyclin D1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16976-50, BZ16976-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: P24385

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-P, IP,

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20

Background: Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 34 kDa; Observed MW: 34 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Cyclin D1 in 3T3 lysates using Cyclin D1 antibody., Western blot analysis of Cyclin D1 in MCF-7, C6, PC-12 lysates using Cyclin D1 antibody., Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Cyclin D1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: CCND1; BCL1; D11S287E; PRAD1; U21B31; Cyclin D1

Immunogen: A synthetic peptide of human Cyclin D1

Conjugate: Unconjugated

Modification: Unmodified