Cyclin D1 Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A11022-20, A11022-100
Citations, Manuals and MSDS Available upon request.
Background: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of human cancers.
Category: Polyclonal Antibodies
Applications: WB, IHC-P, IF/ICC, IP, ELISA
Cross-reactivity: Human, Mouse, Rat
Protein Weight: 34kDa
Observed Molecular Weight: 34 kDa
Immunogen: Recombinant protein (or fragment)
Species: Human
GeneID: 595
SWISS: P24385
Alternate Names: BCL1; PRAD1; U21B31; D11S287E; Cyclin D1
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH 7.3.
Recommended Dilutions: WB, 1:1000 - 1:2000 IHC-P, 1:50 - 1:200 IF/ICC, 1:50 - 1:200 IP, 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Research Areas: Epigenetics Nuclear Signaling, Protein phosphorylation, Cancer, Signal Transduction, PI3K-Akt Signaling Pathway, Cell Biology Developmental Biology, Cell Cycle, Cell Cycle Control-G1 S Checkpoint.
NCBI Alias: CCND1
Research Use Only