Cyclin D3 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90357-50, BS90357-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P30281(Human) P30282(Mouse)
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB, ICC/IF FC
All Applications: WB:1:1,000
ICC:1:50-1:200
FC:1:10-1:50
Background: The proliferation of eukaryotic cells is controlled at specific points in the cell cycle, particularly at the G1 to S and the G2 to M transitions. It is well established that the Cdc2 p34-cyclin B protein kinase plays a critical role in the G2 to M transition while cyclin A associates with Cdk2 p33 and functions in S phase. Considerable effort directed towards the identification of G1 cyclins has led to the isolation of cyclin D, cyclin C and cyclin. Of these, cyclin D corresponds to a putative human oncogene, designated PRAD1, which maps at the site of the Bcl-1 rearrangement in certain lymphomas and leukemias. Two additional human type D cyclins, as well as their mouse homologs, have been identified. Evidence has established that members of the cyclin D family function to regulate phosphorylation of the retinoblastoma gene product, thereby activating E2F transcription factors.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Cyclin D3 polyclonal antibody detects endogenous levels of Cyclin D3 protein.
Bioworld Molecular Weight: 32 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Cyclin D3 on different lysates using anti-Cyclin D3 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Jurkat
Lane 2: Hela, ICC staining Cyclin D3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: CCND3, CCND 3, Ccnd3, CCND3, CyclinD3, D3 type cyclin, G1 S specific cyclin D3, G1/S specific cyclin D3, G1/S-specific cyclin-D3,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified