Product Description

Cyclin E1 (I119) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1085-50, BS1085-100

Sizes: 50µl, 100µl

Swiss-Prot: P24864

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC, IF

Background: Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Cyclin E1 (I119) polyclonal antibody detects endogenous levels of Cyclin E1 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Cyclin E1 (I119) pAb at 1:500 dilution
Lane 1: HEK293T whole cell lysate (20ug)
Lane 2: SK-OVCAR3 whole cell lysate (20ug)
Lane 3: Hela whole cell lysate (20ug)
Lane 4: PC12 whole cell lysate (20ug)
Lane 5: CT26 whole cell lysate (20ug)
Lane 6: The Testis tissue lysate of Rat (20ug), Immunohistochemistry (IHC) analyzes of Cyclin E1 (I119) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 49 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: G1/S-specific cyclin-E1; CCNE1; CCNE

Immunogen: Synthetic peptide, corresponding to amino acids 90-135 of Human Cyclin E1.

Conjugate: Unconjugated

Modification: Unmodified