Product Description

Cyclin E1 (phospho-T395) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS4057-50, BS4057-100

Swiss-Prot: P24864

Host: Rabbit

Cyclin E1 (phospho-T395) Polyclonal Antibody-BS4057

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-Cyclin E1 (T395) polyclonal antibody detects endogenous levels of Cyclin E1 protein only when phosphorylated at Thr395

Bioworld Molecular Weight: ~ 48 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of p-Cyclin E1 (T395) pAb at 1:500 dilution
Lane 1: A549 whole cell lysate (40ug)
Lane 2: HepG2 whole cell lysate (40ug)
Lane 3: C6 whole cell lysate (40ug)
Lane 4: BV2 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of p-Cyclin E1 (T395) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.

Alternative Name: G1/S-specific cyclin-E1; CCNE1; CCNE

Immunogen: Synthetic phosphopeptide derived from human Cyclin E1 around the phosphorylation site of Threonine 395.

Modification: Phosphorylation

Conjugate: Unconjugated