Cyclin E1 (Phospho-T77) Polyclonal Antibody-BS94055

Cyclin E1 (Phospho-T77) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS94055-50, BS94055-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P24864(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC/IF, IHC

All Applications: WB:1:1,000
ICC:1:50-1:200
IHC:1:50-1:200

Background: Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner. The cyclin E/E2F complex is detected primarily during the G1 phase of the cell cycle and decreases as cells enter S phase. E2F is known to be a critical transcription factor for expression of several S phase specific proteins.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Cyclin E1 (Phospho-T77) polyclonal antibody detects endogenous levels of Cyclin E1 protein only when phosphorylated at T77.

Bioworld Molecular Weight: 48 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: ICC staining Phospho-Cyclin E1(T77) in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., ICC staining Phospho-Cyclin E1(T77) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: CCNE1, CCNE, Ccne1, CCNE1, cyclin E variant ex5del, cyclin E variant ex7del, Cyclin E1, Cyclin Es, Cyclin Et, CyclinE, G1/S specific cyclin E, G1/S-specific cyclin-E1,

Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Thr77 of human Cyclin E1.

Conjugate: Unconjugated

Modification: Phosphorylation