Cyclin E1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90358-50, BS90358-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P24864(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, ICC/IF
All Applications: WB:1:1,000
ICC:1:50-1:200
Background: Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner. The cyclin E/E2F complex is detected primarily during the G1 phase of the cell cycle and decreases as cells enter S phase. E2F is known to be a critical transcription factor for expression of several S phase specific proteins.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Cyclin E1 polyclonal antibody detects endogenous levels of Cyclin E1 protein.
Bioworld Molecular Weight: 48 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: ICC staining Cyclin E1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., ICC staining Cyclin E1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: CCNE1, CCNE, Ccne1, CCNE1, cyclin E variant ex5del, cyclin E variant ex7del, Cyclin E1, Cyclin Es, Cyclin Et, CyclinE, G1/S specific cyclin E, G1/S-specific cyclin-E1,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified