Product Description

Cyclin E1 (S73) Polyclonal Antibody

Catalogue Numbers: BS1742-50, BS1742-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P24864

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Cyclin E1 (L389) polyclonal antibody detects endogenous levels of Cyclin E1 protein.

Extra Notes: Western blot (WB) analysis of Cyclin E1 (S73) pAb at 1:500 dilution
Lane1:HEK293T whole cell lysate(40µg)
Lane2:MCF-7 whole cell lysate(40µg)
Lane3:Myla2059 whole cell lysate(40µg)
Lane4:The testis tissue lysate of Rat(40µg)
Lane5:CT-26 whole cell lysate(40µg)

Bioworld Molecular Weight: ~ 48 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: G1/S-specific cyclin-E1; CCNE1; CCNE

Immunogen: Synthetic peptide, corresponding to amino acids 360-400 of Human Cyclin E1.

Conjugate: Unconjugated

Modification: Unmodified