Product Description

Cyclin G (I194) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1088-50, BS1088-100

Sizes: 50µl, 100µl

Swiss-Prot: P51959

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Cyclins are the regulatory subunits of Cdc2 p34 and related cyclin-dependent kinases (Cdks) which play critical roles in the control of cell cycle progression. The catalytic subunit for cyclin A and B is Cdc2 p34 kinase. The Cdc2- cyclin B complex controls the G2 to M transition whereas Cdc2-cyclin A regulates S phase progression. The G1 to S transition, however, appears to be controlled by the G1 cyclins. Cyclin D1 accumulates during G1 and associates with Cdk2, Cdk4 and Cdk5. Cyclin E and Cdk2 interact during the G1 to S transition. Cyclin G contains a typical N terminal cyclin box and a carboxy terminal domain sequence homologous to the tyrosine phosphorylation site of the epidermal growth factor receptor. Cyclin G expression is induced within 3 hours after growth stimulation and remains elevated with no apparent cell cycle dependency. Cyclin G2 shares 53% amino acid sequence identity with cyclin G1. Peak expression of cyclin G2 is seen in late S phase, as opposed to cyclin G1 expression, which is constitutive.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Cyclin g (I194) polyclonal antibody detects endogenous levels of Cyclin G protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Cyclin G (I194) polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate (40ug)
Lane 2: HCT116 whole cell lysate (40ug)
Lane 3: SK-OVCAR3 whole cell lysate (40ug)
Lane 4: CT26 whole cell lysate (40ug)
Lane 5: PC12 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Cyclin G (I194)pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 29 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Cyclin-G1; Cyclin-G; CCNG1; CCNG; CYCG1

Immunogen: Synthetic peptide, corresponding to amino acids 180-220 of Human Cyclin G.

Conjugate: Unconjugated

Modification: Unmodified