Product Description

Cyclin H (Phospho-T315) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS64522-50, BS64522-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P51946

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB:1:500~1:1000 IHC:1:50~1:200

Background: Cyclin H belongs to the highly conserved cyclin family, and are regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin H forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, and thus functions as a CDK-activating kinase (CAK). Cyclin H and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Cyclin H (Phospho-T315) polyclonal antibody detects endogenous levels of Cyclin H protein only when phosphorylated at Thr315.

Bioworld Molecular Weight: ~ 37 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Cyclin H (Phospho-T315) polyclonal antibody at 1:500 dilution
Lane A: Hela whole cell lysate
Lane B: H1688 whole cell lysate
Lane C: The Liver tissue lysate of Mouse
Lane D: The Liver tissue lysate of Rat

Alternative Name: Cyclin-H; MO15-associated protein; p34; p37; CCNH

Immunogen: Synthetic phosphopeptide derived from human Cyclin H around the phosphorylation site of Threonine 315.

Conjugate: Unconjugated

Modification: Phosphorylation