Product Description

Cyclin H Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90360-50, BS90360-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P51946(Human) Q61458(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, ICC/IF, IHC, IP, , FC

All Applications: WB:1:1,000-1:5,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100

Background: Progression through the cell cycle requires activation of a series of enzymes designated cyclin dependent kinases (Cdks). The monomeric catalytic subunit, Cdk2, a critical enzyme for initiation of cell cycle progression, is completely inactive. Partial activation is achieved by the binding of regulatory cyclins such as cyclin D1, while full activation requires, in addition, phosphorylation at Thr-160. The enzyme responsible for phosphorylation of Thr-160 in Cdk2 and also Thr-161 in Cdc2 p34, designated Cdk-activating kinase (CAK), has been partially purified and shown to be comprised of a catalytic subunit and a regulatory subunit. The catalytic subunit, designated Cdk7, has been identified as the mammalian homolog of MO15, a protein kinase demonstrated earlier in starfish and Xenopus. The regulatory subunit is a novel cyclin (cyclin H) and is required for activation of Cdk7. Like other Cdks, Cdk7 contains a conserved threonine required for full activity; mutation of this residue severely reduces CAK activity.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Cyclin H polyclonal antibody detects endogenous levels of Cyclin H protein.

Bioworld Molecular Weight: 38 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Cyclin H on K562 cells lysates using anti-Cyclin H antibody at 1/1,000 dilution., ICC staining Cyclin H in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: CCNH, 6330408H09Rik, AI661354, AV102684, AW538719, CAK, CAK complex subunit, ccnh, CCNH, CDK activating kinase, CDK activating kinase complex subunit, Cyclin dependent kinase activating kinase, cyclin dependent kinase activating kinase complex subunit, Cyclin H, Cyclin-H, CyclinH, MO15 associated protein, MO15-associated protein, p34, p36, p37,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified