Product Description

Cyclin L1 (E491) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1285-50, BS1285-100

Sizes: 50µl, 100µl

Swiss-Prot: Q9UK58

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Cell proliferation is controlled at specific stages of the cell cycle by distinct protein kinase complexes. These complexes consist of a catalytic subunit associating with a specific regulatory subunit to form the active kinase. The cyclins, which include cyclin A, B, C, D, E, F, G, H, I, K, L, T and their related proteins, including Dbf4, comprise the regulatory subunits of these kinase complexes. The controlled activation of the kinase complexes at various intervals of the cell cycle is regulated by the availability of the cyclins to the catalytic subunit. Unlike the catalytic subunit, which is expressed continually, the expression and stability of the regulatory subunit fluctuates depending on the stage of the cell cycle and, thereby, regulates the kinase activity. Cyclin L1 is a ubiquitously expressed nuclear protein that can be detected in higher levels in thymus. In neck and head squamous cell carcinomas, cyclin L1 can be overexpressed and is therefore often considered a proto-oncogene. It interacts with POLR2A, CDC2L and SFRS2. Cyclin L1 plays a role in the mRNA splicing process regulation.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Cyclin-L1 (E491) polyclonal antibody detects endogenous levels of Cyclin-L1 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Cyclin L1 (E491) polyclonal antibody at 1:500 dilution
Lane 1: The thymus tissue lysate of Mouse (40ug)
Lane 2: The thymus tissue lysate of Rat (40ug), Immunohistochemistry (IHC) analyzes of Cyclin L1 (E491) pAbin paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 60 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Cyclin-L1; Cyclin-L; CCNL1; BM-001; UNQ530/PRO1073

Immunogen: Synthetic peptide, corresponding to amino acids 460-518 of Human Cyclin L1.

Conjugate: Unconjugated

Modification: Unmodified