Cyclophilin A Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90361-50, BS90361-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P62937(Human) P17742(Mouse) P10111 (Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
All Applications: WB:1:1,000-1:5,000
IHC:1:50-1:200
Background: The immunosuppressant cyclosporin A (CsA) forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity. Cyclophilins are conserved, ubiquitous and abundant cytosolic peptidyl-prolyl cis-trans isomerases that accelerate the isomerization of XaaPro peptide bonds and the refolding of proteins. Human cyclophilin A (CyPA), an intracellular protein of 165 amino acids, is the target of the CsA and is encoded by a single unique gene conserved from yeast to humans. CyPA is known for its involvement in T cell differentiation and proliferation and is highly expressed in brain. CyPA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication. Cyclophilin B (CyPB) is a member of the cyclophilin family with specific N- and C-terminal extensions. Unlike CyPA, CyPB has a signal sequence leading to its translocation in the endoplasmic reticulum. CyPB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes (9,10).
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Cyclophilin A polyclonal antibody detects endogenous levels of Cyclophilin A protein.
Bioworld Molecular Weight: 18 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Cyclophilin A on different cells lysates using anti-Cyclophilin A antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: A431
Lane 3: HepG2, Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cyclophilin A antibody. Counter stained with hematoxylin.
Alternative Name: PPIase A, PPIA, Cyclophilin A, Cyclophilin, CyclophilinA, Cyclosporin A binding protein, Cyclosporin A-binding protein, CYPA, CYPH, Epididymis secretory sperm binding protein Li 69p, HEL S 69p, MGC117158, MGC12404, MGC23397, Peptidyl prolyl cis trans isomerase A, Peptidyl-prolyl cis-trans isomerase A, Peptidylprolyl isomerase A (cyclophilin A), Peptidylprolyl isomerase A, PPIA, PPIA protein, PPIA, PPIase A, Rotamase A, RotamaseA, T cell cyclophilin,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified