CYP17A1 (N249) Polyclonal Antibody-BS2187

CYP17A1 (N249) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2187-50, BS2187-100

Sizes: 50µl, 100µl

Swiss-Prot: P05093

Host: Rabbit

Reactivity: Human

Applications: WB, IHC, IF

Background: CYP17A (17α-hydroxylase/17,20-lyase) is important for the conversion of pregnenolone and progesterone to dehydroepiandrosterone (DHEA) and androstenedione. In this process, it catalyzes both the 17α-hydroxylation and the 17,20-lyase reaction.CYP17A1 is crucial during sexual development, both during fetal development and during puberty, and is intracellularly regulated by cAMP levels. Defects in the CYP17A1 gene, which encodes for the protein, may cause adrenal hyperplasia type V (AH-V) which is characterized by hypokalemia and hypertension.Male patients affected by AH-V do not undergo normal sexual differentiation and develop female external genitalia and do not undergo pubertal develoment.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: CYP17A1 (N249) polyclonal antibody detects endogenous levels of CYP17A1 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CYP17A1 (N249) pAb at 1:500 dilution
Lane 1: MCF-7 whole cell lysate (40ug)
Lane 2: A2780 whole cell lysate (40ug)
Lane 3: PC3 whole cell lysate (40ug)
Lane 4: HEK293T whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of CYP17A1 (N249) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 57 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Steroid 17-alpha-hydroxylase/17,20 lyase; 17-alpha-hydroxyprogesterone aldolase; CYPXVII; Cytochrome P450 17A1; Cytochrome P450-C17; Cytochrome P450c17; Steroid 17-alpha-monooxygenase; CYP17A1; CYP17; S17AH

Immunogen: Synthetic peptide, corresponding to amino acids 211-260 of Human CYP17A1.

Conjugate: Unconjugated

Modification: Unmodified