CYP17A1 (N249) Polyclonal Antibody-BSP2187

CYP17A1 (N249) Polyclonal Antibody

Catalogue Numbers: BSP2187-50, BSP2187-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: P05093

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB: 1:2000~1:5000

Background: CYP17A1, also known as cytochrome P450C17, is a steroidogenic enzyme belonging to the P450 cytochrome superfamily of monooxygenases. In humans, CYP17A1 expression is abundantly expressed in the adrenal cortex, where it plays a central role in the androgen synthesis pathway (2). CYP17A1 is the primary target of abiraterone, a synthetic steroid used in the treatment of castration-resistant prostate cancer (CRPC). Abiraterone is converted to the more active form D4A, which antagonizes androgen receptor signaling by inhibiting CYP17A1 and other steroidogenic enzymes. This suppresses the synthesis of 5α-dihydrotestosterone (DHT), which is a driver of castration-resistant prostate cancer cell growth.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CYP17A1 (N249) polyclonal antibody detects endogenous levels of CYP17A1 protein.

Extra Notes: Western blot (WB) analysis of CYP17A1 (N249) polyclonal antibody at 1:2000 dilution
Lane1:HEK293T whole cell lysate(40ug)
Lane2:A549 whole cell lysate(40ug)
Lane3:K562 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 60 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Steroid 17-alpha-hydroxylase/17,20 lyase; 17-alpha-hydroxyprogesterone aldolase; CYPXVII; Cytochrome P450 17A1; Cytochrome P450-C17; Cytochrome P450c17; Steroid 17-alpha-monooxygenase; CYP17A1; CYP17, S17AH

Immunogen: Synthetic peptide, corresponding to Human CYP17A1.

Conjugate: Unconjugated

Modification: Unmodified