CYP17A1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90363-50, BS90363-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P05093(Human)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IF
All Applications: WB 1:500 - 1:2000 IF 1:50 - 1:200
Background: The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. P450 enzymes are classified into subfamilies, such as CYP1A, CYP2A, CYP2C, CYP2D, CYP4A14, CYP7A, CYP7B, CYP8B, CYP11A, CYP17A1, CYPI9 and CYP27A, based on sequence similarities. CYP17A (17alpha-hydroxylase/17,20-lyase) is important for the conversion of pregnenolone and progesterone to dehydroepiandrosterone (DHEA) and androstenedione. In this process, it catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. CYP17A1 is crucial during sexual development, both during fetal development and during puberty, and is intracellularly regulated by cAMP levels. Defects in the CYP17A1 gene, which encodes for the protein, may cause adrenal hyperplasia type V (AH-V) which is characterized by hypokalemia and hypertension. Male patients affected by AH-V do not undergo normal sexual differentiation and develop female external genitalia and do not undergo pubertal develoment.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: CYP17A1 polyclonal antibody detects endogenous levels of CYP17A1 protein.
Bioworld Molecular Weight: 57 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: ICC staining Cytochrome P450 17A1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., ICC staining Cytochrome P450 17A1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: Cytochrome P450c17, CYP17A1, CYP17, S17AH, 20 lyase, CP17A, CPT7, CYP17, CYP17A1, CYPXVII, Cytochrome P450 17A1, Cytochrome P450 family 17, Cytochrome P450 family 17 subfamily A polypeptide 1, Cytochrome p450 subfamily XVII (steroid 17 alpha hydroxylase) adrenal hyperplasia, Cytochrome p450 XVIIA1, Cytochrome P450-C17, Cytochrome P450c17, OTTHUMP00000020382, P450 C17, P450c17, S17AH, Steroid 17 alpha hydroxylase/17, 20 lyase, Steroid 17 alpha monooxygenase, Steroid 17-alpha-hydroxylase/17, Steroid 17-alpha-monooxygenase,
Immunogen: Recombinant protein
Conjugate: Unconjugated
Modification: Unmodified