Product Description

CYP1B1 Polyclonal Antibody

Catalogue Numbers: BS5681-50, BS5681-100

Sizes: 50µl, 100µl

Alternative Name: Cytochrome P450 1B1; CYPIB1; CYP1B1

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q16678

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB ICC

All Applications: WB: 1:500~1:1000
ICC: 1:50~1:200

Background: Cytochrome P450 1B1 (CYP1B1) is a key enzyme involved in the production of potentially carcinogenic estrogen metabolites and the activation of environmental carcinogens and is the predominant member of the CYP1 family expressed in normal breast tissue and breast cancer. Estrogen has been proposed to trigger breast cancer development via an initiating mechanism involving its metabolite, catechol estrogen (CE). CYP1B1 catalyzes the conversion of 17-beta-estradiol to the catechol estrogen metabolites 2-OH-E2 and 4-OH-E2 which have both been postulated to be involved in mammary carcinogenesis. Genetic polymorphisms in CYP1B1 may play an important role in human prostate carcinogenesis as well. Polymorphism of the CYP1B1 gene at codon 432 (Val-->Leu) is associated with a change in catalytic function.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CYP1B1 polyclonal antibody detects endogenous levels of CYP1B1 protein.

Bioworld Molecular Weight: ~ 61, 70 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CYP1B1 polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: Raw264.7 cell lysate
Lane 3: PC12 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 452-497 of Human CYP1B1.

Conjugate: Unconjugated

Modification: Unmodified