CYP2A7 Polyclonal Antibody-BS5683

CYP2A7 Polyclonal Antibody

Catalogue Numbers: BS5683-50, BS5683-100

Sizes: 50µl, 100µl

Alternative Name: Cytochrome P450 2A7; CYPIIA7; Cytochrome P450 IIA4

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P20853

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHCICC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
ICC: 1:50~1:200

Background: The cytochrome P450 proteins (CYPs) are monooxygenases that catalyze reactions involved in both drug metabolism and in the synthesis of cholesterol, steroids and other lipids. P450 enzymes are classified into subfamilies, such as CYP1A and CYP2A, based on their sequence similarities. CYP2A7 (cytochrome P450 2A7), also known as CPA7, CPAD, CYPIIA7 or P450-IIA4, is a 494 amino acid peripheral membrane protein that belongs to the CYP2A subfamily of cytochrome P450 proteins. Localized to the endoplasmic reticulum, CYP2A7 can be induced to high levels in liver in response to foreign compounds, such as carcinogens, pesticides and drugs. CYP2A7 functions as a hydroxylase that uses a heme group to catalyze the oxidation of target flavoproteins.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CYP2A7 polyclonal antibody detects endogenous levels of CYP2A7 protein.

Bioworld Molecular Weight: ~ 56 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CYP2A7 polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: PC12 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 435-480 of Human CYP2A7.

Conjugate: Unconjugated

Modification: Unmodified