Product Description

CYP2E1 Polyclonal Antibody

Catalogue Numbers: BS5686-50, BS5686-100

Sizes: 50µl, 100µl

Alternative Name: Cytochrome P450 2E1; 4-nitrophenol 2-hydroxylase; CYPIIE1; Cytochrome P450-J; Cytochrome P450 2E1, N-terminally processed; CYP2E

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P05181

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB ICC

All Applications: WB: 1:500~1:1000
ICC: 1:50~1:200

Background: Cytochrome P450 2E1 (CYP2E1) localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous levels of substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. CYP2E1 plays an important role in alcohol metabolism and participates in the metabolic activation of various carcinogens. Chronic ethanol consumption results in the induction of hepatic CYP2E1 in humans, which may play an important role in the pathogenesis of alcoholic liver disease. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CYP2E1 polyclonal antibody detects endogenous levels of CYP2E1 protein.

Bioworld Molecular Weight: ~ 57 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CYP2E1 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T cell lysate
Lane 2: NIH-3T3 cell lysate
Lane 3: H9C2 cell lysate

Immunogen: Synthetic peptide, corresponding to the 411-456 of Human CYP2E1.

Conjugate: Unconjugated

Modification: Unmodified