Product Description

CYP2J2 Polyclonal Antibody

Catalogue Numbers: BS5687-50, BS5687-100

Sizes: 50µl, 100µl

Alternative Name: Cytochrome P450 2J2; Arachidonic acid epoxygenase; CYPIIJ2

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P51589

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHCICC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
ICC: 1:50~1:200

Background: CYP2J2 (cytochrome P450 2J2), also known as CPJ2, is a member of the cytochrome P450 protein superfamily. Localized to the ER (endoplasmic reticulum) and microsomal membranes, CYP2J2 is one of three cytochrome P450 enzymes that are responsible for metabolizing arachidonic acid to epoxyeicosatrienoic acids. Functioning via an NADPH-dependent olefin epoxidation, CYP2J2 epoxidizes endogenous cardiac arachidonic acid pools to four different isoforms of epoxyeicosatrienoic acid, all of which are important regulators of cardiovascular homeostasis and vascular tone. CYP2J2 is highly expressed in the heart with low levels of expression found in the liver, colon and kidneys. Up-regulation of CYP2J2 by a c-Jun responsive pathway is thought to promote the neoplastic phenotype of certain carcinoma cells, implicating CYP2J2 in carcinogenesis.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CYP2J2 polyclonal antibody detects endogenous levels of CYP2J2 protein.

Bioworld Molecular Weight: ~ 57 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CYP2J2 polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: NIH-3T3 cell lysate
Lane 3: H9C2 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 270-315 of Human CYP2J2.

Conjugate: Unconjugated

Modification: Unmodified