Product Description

CYP39A1 Polyclonal Antibody

Catalogue Numbers: BS62104-50, BS62104-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: Q9NYL5

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB

All Applications: WB: 1:500~1:1000

Background: 24-hydroxycholesterol 7-alpha-hydroxylase, a cytochrome P450 monooxygenase involved in neural cholesterol clearance through bile acid synthesis (PubMed:25201972, PubMed:10748047). Catalyzes 7-alpha hydroxylation of (24S)-hydroxycholesterol, a neural oxysterol that is metabolized to bile acids in the liver (PubMed:25201972, PubMed:10748047). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CYP39A1 polyclonal antibody detects endogenous levels of CYP39A1 protein.

Extra Notes: Western blot (WB) analysis of CYP39A1 polyclonal antibody at 1:1000 dilution
Lane1:C6 whole cell lysate(40ug)
Lane2:AML-12 whole cell lysate(40ug)
Lane3:PC3 whole cell lysate(40ug)
Lane4:Pancl whole cell lysate(40ug)
Lane5:HEK293T whole cell lysate(40ug)
Lane6:HepG2 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 55 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: 24-hydroxycholesterol 7-alpha-hydroxylase; Cytochrome P450 39A1; hCYP39A1; Oxysterol 7-alpha-hydroxylase; CYP39A1

Immunogen: Synthetic peptide, corresponding to Human CYP39A1.

Conjugate: Unconjugated

Modification: Unmodified