Product Description

CYP4F2 (M66) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS9135-50, BS9135-100

Sizes: 50µl, 100µl

Swiss-Prot: P78329

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: CYP4F isoforms are involved in the oxidation of important cellular mediators, such as leukotriene B4 (LTB4) and prostaglandins. CYP4F2 catalyzes ω-hydroxylation of LTB4 to a less potent proinflammatory eicosanoid, 20-OH-LTB4, as well as arachidonic acid. CYP4F2 is expressed in liver and kidney. Its expression is repressed by peroxisomal proliferators and induced by retinoic acid. X-linked adrenoleukodystrophy (X-ALD) is a severe neurodegenerative disorder biochemically characterized by elevated levels of very long-chain fatty acids (VLCFA). CYP4F2 participates in the ω-hydroxylation of VLCFAs, which may provide an alternative mode of treatment for X-ALD patients.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: CYP4F2 (M66) polyclonal antibody detects endogenous levels of CYP4F2 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CYP4F2 polyclonal antibody at 1:1000 dilution Lane 1: HEPG2 whole cell lysate (55ug)
Lane 2: LO2 whole cell lysate (38ug) Lane 3: The liver tissue lysate of Rat (41ug) Lane 4: The liver tissue lysate of Mouse (38ug) , Immunohistochemistry (IHC) analyzes of CYP4F2 (M66) pAb in paraffin-embedded human liver cancer tissue.

Bioworld Molecular Weight: ~ 60 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Cyp4f14; CYPIVF1; Cytochrome P450 LTB omega; Cytochrome P450; family 4; subfamily F; polypeptide 2; EC 1.14.14.1; Leukotriene b (4) 20 monooxygenase; Leukotriene b (4) omega hydroxylase; MGC126966; MGC93476; P450 A3;

Immunogen: Synthetic peptide, corresponding to amino acids 36-90 of Human CYP4F2.

Conjugate: Unconjugated

Modification: Unmodified