Product Description

CYP4F2 Polyclonal Antibody

Catalogue Numbers: BS5690-50, BS5690-100

Sizes: 50µl, 100µl

Alternative Name: Leukotriene-B(4) omega-hydroxylase 1; CYPIVF2; Cytochrome P450 4F2; Cytochrome P450-LTB-omega; Leukotriene-B(4) 20-monooxygenase 1

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P78329

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHCICC IP

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
ICC: 1:50~1:200
IP: 1:50~1:200

Background: CYP4F isoforms are involved in the oxidation of important cellular mediators, such as leukotriene B4 (LTB4) and prostaglandins. CYP4F2 catalyzes omega-hydroxylation of LTB4 to a less potent proinflammatory eicosanoid, 20-OH-LTB4 as well as arachidonic acid. CYP4F2 is expressed in liver and kidney. Its expression is repressed by peroxisomal proliferators and induced by retinoic acid. X-linked adrenoleukodystrophy (X-ALD) is a severe neurodegenerative disorder biochemically characterized by elevated levels of very long-chain fatty acids (VLCFA). CYP4F2 participates in the omega-hydroxylation of VLCFAs, which may provide an alternative mode of treatment for X-ALD patients.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CYP4F2 polyclonal antibody detects endogenous levels of CYP4F2 protein.

Bioworld Molecular Weight: ~ 60 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CYP4F2 polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: NIH-3T3 cell lysate
Lane 3: PC12 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 76-121 of Human CYP4F2.

Conjugate: Unconjugated

Modification: Unmodified