Product Description

CYP7B1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS77542-50, BS77542-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O75881

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:1000 | IF/ICC,1:50 - 1:200

Background: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 55KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of A-431, using CYP7B1 antibody at 1:1980 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s., Immunofluorescence analysis of NIH/3T3 cells using CYP7B1 Rabbit pAb at dilution of 1:25. Blue: DAPI for nuclear staining., Immunofluorescence analysis of PC-12 cells using CYP7B1 Rabbit pAb at dilution of 1:25. Blue: DAPI for nuclear staining.

Alternative Name: CYP7B1; CBAS3; CP7B; SPG5A

Immunogen: A synthetic peptide of human CYP7B1(NP_004811.1).

Conjugate: Unconjugated

Modification: Unmodified