Product Description

Cystatin C (F122) Polyclonal Antibody

Catalogue Numbers: BS3973-50, BS3973-100

Sizes: 50µl, 100µl

Alternative Name: Cystatin-C; Cystatin-3; Gamma-trace; Neuroendocrine basic polypeptide; Post-gamma-globulin; CST3

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P01034

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Cystatin C is a cysteine (thiol) protease inhibitor that belongs to the type II cystatin gene superfamily and is the most abundant extracellular inhibitor of cysteine proteases. Cystatin C is a constitutively secreted, amyloidogenic protein, which forms a two-fold symmetric dimer and modulates both cysteine protease activity and the expression of class II MHC molecules. Expression of cystatin C is an indicator of kidney function and glomerular filtration rate. Mutations in the cystatin C gene can lead to protein aggregates, which are implicated in hereditary amyloid angiopathy (HCCAA) and cerebral hemorrhage. Although both wild-type and mutant cystatin C are capable of forming concentration dependent inactive dimers, mutant cystatin C dimerizes at lower concentrations and is more susceptible to serine proteases, which may facilitate aggregation. In neuronal cells, oxidative stress stimulates expression of cystatin C, which may positively regulate apoptosis.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Cystatin C (F122) polyclonal antibody detects endogenous levels of Cystatin C protein =

Bioworld Molecular Weight: ~ 16 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Cystatin C (F122) pAb at 1:1000 dilution
Lane 1: PC3 whole cell lysate(20ug)
Lane 2: The Brain tissue lysate of Mouse(40ug)
Lane 3: C6 whole cell lysate(40ug)
Lane 4: HepG2 whole cell lysate(40ug)
Lane 5: H1792 whole cell lysate(40ug)
Lane 6: HEK293T whole cell lysate(40ug)
Lane 7: MCF-7 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 101-150 of Human Cystatin C.

Conjugate: Unconjugated

Modification: Unmodified