Product Description

Cystatin C Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90369-50, BS90369-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P01034(Human) P21460(Mouse) P14841(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB:1:1,000-1:2,000
IHC:1:50-1:200

Background: Cystatin C is a cysteine (thiol) protease inhibitor that belongs to the type II cystatin gene superfamily and is the most abundant extracellular inhibitor of cysteine proteases. Cystatin C is a constitutively secreted, amyloidogenic protein, which forms a two-fold symmetric dimer and modulates both cysteine protease activity and the expression of class II MHC molecules. Expression of cystatin C is an indicator of kidney function and glomerular filtration rate. Mutations in the cystatin C gene can lead to protein aggregates, which are implicated in hereditary amyloid angiopathy (HCCAA) and cerebral hemorrhage. Although both wild-type and mutant cystatin C are capable of forming concentration dependent inactive dimers, mutant cystatin C dimerizes at lower concentrations and is more susceptible to serine proteases, which may facilitate aggregation. In neuronal cells, oxidative stress stimulates expression of cystatin C, which may positively regulate apoptosis.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Cystatin C polyclonal antibody detects endogenous levels of Cystatin C protein.

Bioworld Molecular Weight: 16 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Cystatin C on different lysates using anti-Cystatin C antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: Mouse spleen, Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Cystatin C antibody. Counter stained with hematoxylin.

Alternative Name: CST3, AD 8, AD8, Amyloid angiopathy and cerebral hemorrhage, ARMD11, bA218C14.4 (cystatin C), bA218C14.4, Cst 3, Cst3, CST3 protein, Cystatin 3, Cystatin-3, Cystatin-C, Cystatin3, CystatinC, CYTC, Epididymis secretory protein Li 2, Gamma trace, Gamma-trace, HCCAA, HEL S 2, MGC117328, Neuroendocrine basic polypeptide, Post gamma globulin, Post-gamma-globulin,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified