Cytochrome P450 Reductase Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90372-50, BS90372-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P16435(Human) P37040(Mouse) P00388(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC, IF, IHC, FC
All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds. Several P450 enzymes have been classified by sequence similarities as members of the CYP1A and CYP2A subfamilies. CYPOR, also known as cytochrome P450 reductase and NADPH cytochrome P450 reductase, is a microsomal enzyme responsible for the transfer of electrons from NADPH to cytochrome P450 enzymes during the P450 catalytic cycle. CYPOR is localized to the endoplasmic reticulum, where it is also able to transfer electrons to heme oxygenase and cytochrome b5. CYPOR is structurally related to two separate flavoprotein families, ferredoxin nucleotide reductase (FNR) and flavodoxin. Electron transfer of CYPOR requires the binding of two flavin cofactors, FAD and FMN, to the FNR and flavodoxin domains, respectively.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Cytochrome P450 Reductase polyclonal antibody detects endogenous levels of Cytochrome P450 Reductase protein.
Bioworld Molecular Weight: 77 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Cytochrome P450 Reductase on SK-Br-3 (1), A549 (2) and mouse lung (3) lysate using anti-Cytochrome P450 Reductase antibody at 1/500 dilution., ICC staining Cytochrome P450 Reductase in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: CPR, P450R, POR, CYPOR, CPR, CYPOR, Cytochrome p450 oxidoreductase, DKFZp686G04235, FLJ26468, NADPH Cytochrome P450 Reductase, NADPH dependent cytochrome P450 reductase, NADPHcytochrome P450 reductase, NCPR, P450 (cytochrome) oxidoreductase, P450 Cytochrome Oxidoreductase, P450R, por,
Immunogen: Recombinant protein
Conjugate: Unconjugated
Modification: Unmodified