Cytokeratin 16 (S451) Polyclonal Antibody-BS1202

Cytokeratin 16 (S451) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1202-50, BS1202-100

Sizes: 50µl, 100µl

Swiss-Prot: P08779

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Keratin 16 is expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyperproliferation-related keratins). Keratin 16 is absent in normal breast tissue and in noninvasive breast carcinomas. Only 10% of the invasive breast carcinomas show diffuse or focal positivity. Reportedly, a relatively high concordance was found between the carcinomas immunostaining with the basal cell and the hyperproliferation-related keratins, but not between these markers and the proliferation marker Ki-67. This supports the conclusion that basal cells in breast cancer may show extensive proliferation, and that absence of Ki-67 staining does not mean that (tumor) cells are not proliferating

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Cytokeratin 16 (S451) polyclonal antibody detects endogenous levels of Cytokeratin 16 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Cytokeratin 16 (S451) polyclonal antibody at 1:500 dilution
Lane 1: MCF-7 cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: PC12 cell lysate, Immunohistochemistry (IHC) analyzes of Cytokeratin 16 (S451) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 52 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Keratin, type I cytoskeletal 16; Cytokeratin-16; Cytokeratin16; CK-16; CK16; Keratin-16; Keratin 16; Keratin16; Short name=K16; KRT16; KRT16A

Immunogen: Synthetic peptide, corresponding to amino acids 421-470 of Human Cytokeratin 16.

Conjugate: Unconjugated

Modification: Unmodified