Product Description

Cytokeratin 18 Monoclonal Antibody

Catalogue Numbers: MB66811-50, MB66811-100

Sizes: 50µl, 100µl

Product: Mouse IgG3. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: P05783

Host: Mouse

Reactivity: Human

Applications: WB

All Applications: WB (1/500 - 1/1000)

Background: Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and maintain the overall structural integrity of epithelial cells. Cytokeratins are useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. Cytokeratin polypeptides are designated 1 to 20 and are divided into the type I and type II subgroups. The type II family members comprise the basic to neutral cytokeratins 1-8, while the type I group comprises the acidic cytokeratins 9-20. Cytokeratin 5 is expressed in normal basal cells. Mutations of the cytokeratin 5 gene (KRT5) have been shown to result in the autosomal dominant disorder epidermolysis bullosa (EB). Cytokeratin 6 is expressed in suprabasal keratinocytes of wounded epidermis and in acrosyringeal keratinocytes in normal skin. Cytokeratin 18 is one of the first keratins expressed in the embryo, and persists into adult tissues. It is a major component of all simple epithelia (but not of stratified squamous epithelia) and adenocarcinomas.

Purification and Purity: This antibody is purified through a protein G column.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of Cytokeratin 18 protein.

Extra Notes: Western blot analysis of Cytokeratin 18 expression in HepG2 (A), K562 (B), NCIH460 (C) whole cell lysates.

Bioworld Molecular Weight: ~ 50kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: CYK18; Keratin, type I cytoskeletal 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Cytokeratin 18. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified