Product Description

Cytokeratin 20 Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9831M-50, BS9831M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P35900

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, ICC/IF, IHC, IP, , FC

All Applications: WB: 1:1000-1:5000 IHC/ICC/IF: 1:50-1:200
IP: 1:50-1:200 FC: 1:10-1:100

Background: Cytokeratin 20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract, and Cytokeratin 20 is a useful marker of pancreatic and colorectal cancer. Cytokeratin 20 is also helpful in distinguishing different types of highly related carcinomas, such as renal oncocytomas from renal cell carcinomas.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of Cytokeratin 20 and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 48 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Cytokeratin 20 Rabbit mAb at 1:1000 dilution
Lane 1: Lovo whole cell lysate
Lane 2: HCT116 whole cell lysate
Lane 3: CRC whole cell lysate, Immunohistochemical analysis of paraffin-embedded rat small intestine tissue using anti-Cytokeratin 20 antibody. Counter stained with hematoxylin., ICC staining Cytokeratin 20 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Alternative Name: Keratin, type I cytoskeletal 20; Cytokeratin-20; Short name=CK-20; Keratin-20; Short name=K20; Protein IT; KRT20; CK 20; CK20; Keratin 20

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Unmodified