Cytokeratin 7 Monoclonal Antibody-MB66494

Cytokeratin 7 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66494-50, MB66494-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P08729

Host: Mouse

Reactivity: Human

Applications: WB, IF/ICC

All Applications: WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100)

Background: Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments . Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers . Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases .
Keratin 7 and Keratin 19 are present in hepatic and pancreatic progenitor/stem cells .

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of Cytokeratin 7 protein.

Bioworld Molecular Weight: ~ 55 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Cytokeratin 7 expression in T47D (A), Hela (B), HepG2 (C) whole cell lysates., Immunofluorescent analysis of Cytokeratin 7 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Alternative Name: SCL; Keratin type II cytoskeletal 7; Cytokeratin-7; CK-7; Keratin-7; K7; Sarcolectin; Type-II keratin Kb7

Immunogen: Purified recombinant human Keratin 7 protein fragments expressed in E.coli.

Conjugate: Unconjugated

Modification: Unmodified