Product Description

Cytokeratin-7 Mouse Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB9008-50, MB9008-100

Product: Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P08729

Host: Mouse

Reactivity: Human

Applications: WB, IHC

All Applications: WB: 1:1000 IHC: 1:200

Background: Keratin-7 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. Keratin-7 is found in simple glandular epithelia, and in transitional epithelium. Epithelial cells of the lung and breast both contain keratin-7, but some other glandular epithelia, such as those of the colon and prostate, do not. Because the keratin-7 antigen is found in both healthy and neoplastic cells, antibodies to CK7 can be used in immunohistochemistry to distinguish ovarian and transitional cell carcinomas from colonic and prostate cancers, respectively.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of Cytokeratin-7 and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 50 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Cytokeratin-7 Mouse mAb at 1:1000 dilution
Lane 1: MCF7 whole cell lysate
Lane 2: A549 whole cell lysate
Lane 3: Hela whole cell lysate
Lane 4: HepG2 whole cell lysate
Lane 5: SKBR3 whole cell lysate
Lane 6: SKOV3 whole cell lysate
Lane 7: SW1990 whole cell lysate, Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Cytokeratin-7 antibody. Counter stained with hematoxylin.

Alternative Name: Cytokeratin-7; CK-7; Cytokeratin 7; Cytokeratin7; Keratin 7; Keratin7; Keratin-7; KRT7; SCL; Sarcolectin; Type-II keratin Kb7

Immunogen: This antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) corresponding to a region of human Cytokeratin-7.

Conjugate: Unconjugated

Modification: Unmodified